HiTrap IXSelect : pour la purification du facteur de coagulation IX. EstK was purified to homogeneity using standard procedures. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. HiTrap IgY : designed for purification of IgY. An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25☌. Of these, the Q (quaternary ammonium) column is a strong anion exchange column, while DEAE (diethyla- minoethyl) is a weak anion exchange column 8. HiTrap IgM : designed for purification of monoclonal IgM. HiTrap MabSelect™, MabSelect SuRe™ and MabSelect Xtra™ : developed to meet the productivity and sanitisation requirements of industry to develop large-scale antibody purification procedures. HiTrap Protein A and HiTrap Protein G : designed for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants. HiTrap NHS : high-performance medium designed for easy coupling of ligands containing primary amines. HiTrap Capto™ : designed to meet industrial requirements for the production of biological molecules.
It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as requiredĪcronyms and abbreviations : Q = Quaternary ammonium DEAE = DiethylaminoethyleĬm = Carboxymethyle S = Methylsulfonate ANX = Diethylaminopropyle Strong quaternary ammonium (Q) anion exchanger. Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer.HiTrap columns are available in 1 ml and 5 ml bed volume.
0 kommentar(er)
